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KMID : 1134820170460091091
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 9 p.1091 ~ p.1096
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Modification and Validation of Analytical Method for Oxypaeoniflorin and Paeoniflorin in Moutan Cortex Radicis Extract
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Choi Seung-Hyun
Yoo Chang-Kil
Hwang Ji-Hyun
Lee Gi-Bbeum
Lee Young-Jin
Lee Boo-Yong
Lee Ok-Hwan
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Abstract
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The aim of this study was the validation of a modified analytical method for determination of oxypaeoniflorin and paeoniflorin in Moutan Cortex Radicis extract. For validation of the analytical method, we modified established analytical methods and validated improvement. For validation, the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification of oxypaeoniflorin and paeoniflorin were measured by high performance liquid chromatography. The results show that the correlation coefficients of the calibration curve for oxypaeoniflorin and paeoniflorin were 1.0000 and 0.9998, respectively. The LOD for oxypaeoniflorin and paeoniflorin were 0.23 μg/mL and 0.25 μg/mL, respectively. The inter-day and intra-day precision values of oxypaeoniflorin and paeoniflorin were 0.70∼3.19% and 1.74∼2.43%, and 0.32∼0.92% and 0.62∼2.28%, respectively. The inter-day and intra-day accuracies of oxypaeoniflorin and paeoniflorin were 98.33∼102.11% and 97.72∼118.12%, and 98.44∼101.56% and 97.10∼112.00%, respectively. Therefore, the analytical method was validated for the detection of oxypaeoniflorin and paeoniflorin in Moutan Cortex Radicis.
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KEYWORD
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Moutan Cortex Radicis, oxypaeoniflorin, paeoniflorin, HPLC-PDA, method validation
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